Analysis Note

Positive ControlParaventricular nucleus, cortex, or caudate putamen

Application

Frozen Sections (1:1000-1:2000 for free-floating, PAP detection)

General description

This Anti-Neuropeptide Y Rabbit pAb is validated for use in Frozen Sections for the detection of Neuropeptide Y.

Recognizes neuropeptide Y in cortex, caudate, putamen, and paraventricular nucleus.

Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes neuropeptide Y.

Immunogen

porcine neuropeptide Y

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Antibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10^-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Plastic ampoule

Reconstitution

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)